FIGURE 8.

Calcium imaging recordings. (A) Schematic representation of a coronal slice containing the RMTg area. The zone within the square was amplified (right). The dark gray rectangles indicate the region within the RMTg where calcium image recordings were made. (B) Raw data of calcium imaging recordings in control conditions (left) and nicotine (1 μM, right). Each image was obtained from a single frame of the video. (C) Scheme showing the coordinates map of the active cells constructed from the video (see section “Materials and Methods”). The red arrows indicate the locations of the cells recorded in (B). (D) Calcium transients (top) and their first derivate (bottom), which correspond to the cell encircled in (B) (right, red circle). RMTg, rostromedial tegmental nucleus; IPN, interpeduncular nucleus.