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. 2020 Nov 3;10(1):62–73. doi: 10.12997/jla.2021.10.1.62

Fig. 4. Zingerone increases TIMP4 expression. VSMCs were treated with zingerone (100 nM) for 12 hours (A). Cells were treated with or without zingerone and phosphate for 2 days (B, C) mRNA expression of TIMP4 was measured by real-time polymerase chain reaction (A, B). The protein levels of TIMP4 was measured by Western blot analysis (C). Densitometry analysis was measured with the TIMP4 (B, right panel). Data represent the mean±standard error of the mean of 3 individual experiments.

Fig. 4

TIMP, tissue inhibitor of metalloproteinase; VSMC, vascular smooth muscle cell; Pi, inorganic phosphate.

Statistical significance was determined relative to a control by the Student's t-test (*p<0.05; p<0.01) and compared with Pi-treated cells by the Student's t-test (p<0.05).