Table 1. Specific primer for real-time PCR.
| Primer | Sequences (5′→3′) | |
|---|---|---|
| ALP | Forward | AACCCAGACACAAGCATTCC |
| Reverse | GAGAGCGAAGGGTCAGTCAG | |
| Dlx5 | Forward | GCCCACCAACCAGCCAGAGA |
| Reverse | GCGAGGTACTGAGTCTTCTGAAACC | |
| CBFA1 | Forward | AGATGACATCCCCATCCATC |
| Reverse | GTGAGGGATGAAATGCTGG | |
| TIMP1 | Forward | CCAGAGCGATCACTTTGCCT |
| Reverse | GAAAGGTAAACAGTGTTCAGGCT | |
| TIMP2 | Forward | ACGCTTAGCATCACCCAGAAG |
| Reverse | ATAGGGCAGCGTGTGATCTTG | |
| TIMP3 | Forward | ATCCCCAGGATGCCTTCTG |
| Reverse | CCTTCCTTCACCAGCTTCTTT | |
| TIMP4 | Forward | CTAGAAACCAACAGTCAGAAGC |
| Reverse | GTTCTGGTGGTAGTGATGATTC | |
PCR, polymerase chain reaction; ALP, alkaline phosphatase; Dlx5, distal-less homeobox 5; CBFA1, core-binding factor α-1; TIMP, tissue inhibitor of metalloproteinase.