FIGURE 5.

CSAG2 knockdown attenuates CPEB4-mediated paclitaxel resistance in ovarian cancer cells. (A) Cells of SKOV3 (S) and CaOV3 (S) stably expressing empty vector or human CPEB4 were transfected with siRNA targeting control or CSAG2, and further cultured for 3 days. The protein levels of were analyzed by Western blotting. β-Actin was used as a loading control. The representative images are shown. (B) Cells were treated as in (A), and the viable cell number was counted by Trypan blue staining to exclude unviable cells. Data represent mean ± SEM. n = 3. NS, not significant. (C) Cells were treated as in (A), and then treated with 20 nM PTX for indicated periods of time. The viable cell number relative to DMSO treated group is shown. Data represent mean ± SEM. n = 3. **p < 0.01; NS, not significant. (D) Cells were treated as in (A), and then further cultured with 20 nM PTX for 2 weeks. Representative images of cells stained with crystal violet are shown (left). The colony number was quantified by ImageJ (right), and results relative to vector are shown. Data are mean ± SEM. n = 3. **p < 0.01.