Figure 2.
Knockdown of GHET1 inhibits cell proliferation and arrests cell cycle at S phase in Lovo and HCT-116 cells. (A) Real-time PCR analysis for GHET1 in Lovo and HCT-116 cells transfected with negative control sequence (NC) or GHET1-shRNA. (B) MTT assay in Lovo and HCT-116 cells transfected with negative control sequence (NC) or GHET1-shRNA. (C) Flow cytometric analysis for cell cycle in Lovo and HCT-116 cells transfected with negative control sequence (NC) or GHET1-shRNA. Data are presented as mean ± SD. Experiments were independently repeated three times. *p < 0.05, **p < 0.01, ***p < 0.001.
