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. 2021 Jan 13;8:595096. doi: 10.3389/fbioe.2020.595096

FIGURE 2.

FIGURE 2

Isolation and culture of DPSCs. The panel (A) shows that the harvested DPSCs with the typical spindle morphology of mesenchymal cells could maintain a good state of proliferation from passage 0 to 5. Immunofluorescence staining reveals vimentin expression (B), but negative staining for CK-14 in DPSCs (C). Scale bars: 200 μm. P0, passage 0; P1, passage 1; P5, passage 5; T, tissue blocks.