FIGURE 7.

IHC examination of the composites for dental pulp regeneration in vivo. The generated odontoblastic layer–like structures in DPEM/LN group were positive for the related odontogenic markers, Col-1 (A,a), DMP-1 (B,b), DSPP (C,c), and LN (D,d). The expression patterns of native dental pulp tissues are shown in panels (K–N). In DPEM group, the regenerated cellular cementum/bone-like structures are observed in (F–I) without the odontoblastic layer–like structure. As negative control, the harvested samples were negative for PBS (E,J,O). DPEM/LN, laminin-modified DPEM; LN, laminin; Col-1, collagen-1; DMP-1, dentin matrix protein-1; DSPP, dentin sialophosphoprotein; PBS, phosphate buffer solution; (Red arrows label the generated odontoblastic layer–like structure).