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. 2016 Apr 13;23(5):257–266. doi: 10.3727/096504016X14562725373752

Figure 3.

Figure 3

The target relationship between miR-20b and HIF-1α. The target gene was predicted by TargetScan database and identified by luciferase activity report. (A) The wild-type and the mutant HIF-1α 3′-UTR contained the target sequence of miR-20b. (B) MG63 cells were cotransfected with miR-20b mimic or mimic control luciferase reporter vectors containing wild-type (wt) or mutant (mut) HIF-1α 3′-UTR. (C) A similar luciferase assay was performed in MG63 cell lines treated with miR-20b inhibitor or inhibitor control. Luciferase activity was represented as firefly luciferase normalized to renilla luciferase. Data are represented as the mean ± SD of three experiments. *p < 0.05 versus other three groups.