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. 2016 Apr 13;23(5):257–266. doi: 10.3727/096504016X14562725373752

Figure 5.

Figure 5

Overexpression of HIF-1α offsets the suppression effect of miR-20b in MG63 cells. Cells were treated with miR-20b mimic or mimic control with or without HIF-1α overexpression vector. (A) HIF-1α, VEGF, Cdc42, P38, and HSP27 expressions were detected by Western blotting. (B) Relative protein expressions in MG63 cells were quantified using Image-Pro Plus 6.0 software and normalized to β-actin. (C) The number of invading MG63 cells. (D) MTT assay was employed to examine proliferation rates of MG63 cells under similar treatments. The data are shown as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 indicates significantly different.