Figure 5.
TIPE2 overexpression suppresses hypoxia-induced Wnt pathway activation in U251 cells. U251 cells were treated with 100 µM CoCl2 and then incubated for 24 h. (A) The protein expression levels of β-catenin, cyclin D1, and c-myc were determined by Western blot. (B) Quantification analysis was performed using Gel-Pro Analyzer version 4.0 software. The values shown represent the mean ± SD and n = 3 per group. *p < 0.05 compared with the normoxia group. #p < 0.05 compared with the hypoxia group.