H19 and miR-675 are systematically positively correlated with clinical stage in gastric cancer. Relative expression of H19 (A) and miR-675 (B) in different stages of 114 gastric cancer tissues, which were normalized to GAPDH/U6 with respect to normal gastric mucosa. (C) Positive correlation of H19 and miR-675 expression in gastric cancer tissues, r = 0.5725, p < 0.0001, n = 114. (D) Relative H19/miR-675 expression in AGS cells was compared with those of GES-1 cells. (E) AGS cells were transfected with plasmids or oligonucleotides for 48 h. Then the relative expression of H19/miR-675 was validated using quantitative reverse transcription-PCR. Data presented as mean ± SD, n = 3 independent experiments.