Figure 2.

H19/miR-675 promotes proliferation, colony formation, and invasion of AGS cells. (A) AGS cells were plated in a 24-well plate and transfected with plasmids or oligonucleotides, and then the cell number was determined at the indicated time points using a CyQUANT cell proliferation assay. AGS cells, 1 × 10³, treated the same as (A) and were seeded in soft agar for evaluating the ability of colony formation. Representative images show staining of colonies in each treatment (D). The colony formation efficiencies of indicated treatments were summarized (B). Cells were examined for cell invasion in 24-well plates with Transwell chambers. Migrated cells were stained with crystal violet and counted. Six random fields were photographed (shown in E) and summarized (C) per upper chamber in each group. Data presented as mean ± SD, n = 3 independent experiments.