Figure 3.

H19-derived miR-675 regulates RUNX1 and promotes Akt/mTOR phosphorylation. (A) RUNX1 is potentially targeted by miR-675 using bioinformatic analyses. (B) Relative expression of RUNX1 in AGS cells was compared with GES-1 cells. AGS cells were transfected with plasmids or oligonucleotides for 48 h and then the relative expression of RUNX1 was validated using quantitative reverse transcription-PCR (C). Western blotting analyses were performed to detect the protein levels of RUNX1, Akt, and mTOR (D). Data presented as mean ± SD, n = 3 independent experiments.