Table 1.
List of antibodies used in this study.
| ProteinTarget | Dye/Dilution | Secondary antibody* | Use | Company |
|---|---|---|---|---|
| CD45 | APC/1:200 | FACS | eBioscience | |
| CD45 | Pacific blue/1:200 | FACS | Biolegend | |
| CD45 | Cy5/1:200 | FACS | eBioscience | |
| MHCII | PE or PerCp-eF710/1:200 | FACS | Biolegend | |
| Ly6C | PE/Cy7/1:200 | FACS | Biolegend | |
| Ly6G | AlexaFluor647/1:300 | FACS | eBioscience | |
| CD103 | PE/1:200 | FACS | Biolegend | |
| CD11c | PE/Cy7/1:200 | FACS | Biolegend | |
| CD11b | BV711/1:200 | FACS | Biolegend | |
| anti-CD16/32 | 1:50 (blocking) | FACS | Biolegend | |
| Near-IR | APC/Cy7/1:200 | FACS | Thermo Fisher | |
| GFP | 1:600 | AlexaFluor488 | IHC | Novus biologicals |
| MHCII | 1:400 | AlexaFluor647 or Cy3 | IHC | BioLegend |
| βIII-Tubulin | 1:600 | Dylight405 | IHC | BioLegend |
| IBA-1 | 1:200 | AlexaFluor647 | IHC | Abcam |
*All secondary antibodies were raised in donkey and used at 1:800 dilution.