Skip to main content
. 2021 Jan 13;11:581111. doi: 10.3389/fimmu.2020.581111

Table 2.

Primer sequences, primer assays and Taqman probes used for quantitative RT-PCR.

Gene name Primer sequences (Metabion)
forward reverse
EGR-1 5-GAGCGAACA 5-GGCCAGTAT
ACCCTATGAGC-3 AGGTGATGGGA-3
ISG15 5-CCCCAGCAT 5-TGACTGTGA
CTTCACCTTTA-3 GAGCAAGCAGC-3
USP18 5-GTGTCCGTG 5-CTGCAGAAA
ATCTGGTCCTT-3 TACAACGTGCC-3
TLR3 5-GCCTGAATC 5-AGCCCAGAT
ACAATCGCGCACC-3 TATGGGTGCAATCCCT-3
Gene name QuantiTect Primer Assay ID (Qiagen)
IL-1α QT00113505
IFN-β QT00249662
Gene name Taqman Probes Assay ID (Applied Biosystems)
IL-6 Mm00446190
IL-1β Mm00434228
GAPDH Mm99999915_g1