Figure 4.

PCAT6 knockdown inhibited cell invasion of lung cancer cells. CL1-5 and H446 (4 × 10⁵) cells transiently transfected with PCAT6-siRNA were plated in six-well plates; 48 h later, cells were resuspended in serum-free media and seeded into upper Matrigel-precoated Transwell chamber. Medium containing 10% FBS was added in the lower chamber. After 48 h of incubation, the cells that had invaded another side of the membrane were fixed with methanol for 15 min and stained with 0.1% crystal violet for 20 min. Five random fields were photographed (A, B), and the number of invasive cells per chamber was counted (C, D). Data are presented as mean ± SD. **p < 0.01. ***p < 0.0001.