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. 2016 May 11;24(1):25–32. doi: 10.3727/096504016X14575597858609

Figure 5.

Figure 5

SASH1 inhibits the metastatic process of hepatocarcinoma cells through suppressing the Sonic hedgehog signaling pathway. HepG2 cells were transiently transfected with pcDNA3.1-SASH1 or pcDNA3.1 for 48 h. (A) The protein levels of Shh, Smo, Ptc, and Gli-1 were determined by Western blot. (B) Quantitative analysis of protein expression levels of Shh, Smo, Ptc, and Gli-1 using Image-Pro Plus 6.0 software and normalized to GAPDH. The values shown represent the mean ± SD. *p < 0.05 versus pcDNA3.1 group.