Figure 3.

TNF-α is involved in miR-509-mediated suppressive effects on TNBC cells. (A) Western blotting was performed to examine the expression of TNF-α upon transfection with miR-509 in Hs578T cells. (B) Treatment with TNF-α partially abolished the suppressive effects of miR-509 on cell proliferation. (C) Cell apoptosis of Hs578T cells as described after treatment with TNF; TNF induced TNBC cell apoptosis. (D) Transwell assays were conducted to detect the effects on cell invasion of Hs578T cells treated with TNF. TNF increased the cell invasive ability of Hs578T cells.