Skip to main content
. 2016 Oct 27;24(6):495–509. doi: 10.3727/096504016X14685034103950

Figure 4.

Figure 4

Upregulation of c-Myc and DR5 and downregulation of c-FLIPL in CD133hi SNU-475 cells. (A) CD133lo and CD133hi SNU-475 cells were stained with control IgG or anti-DR4 (or anti-DR5) antibody (1:100), and subsequently labeled with FITC-conjugated secondary antibodies (1:100) to determine the surface expression of DR4 and DR5. The cell surface expression was measured by a flow cytometer. Shaded and unshaded peaks correspond to control and specific stainings, respectively. (B) The mRNA and protein levels of c-Myc, DR4/DR5, and c-FLIPL/S were determined by RT-PCR and Western blot, respectively.