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miR-16-1 overexpression suppressed A549 cell proliferation. miR-16-1 mimics, inhibitors, or control was transfected into A549 cells, and then cell proliferation was determined by MTT analysis (A) and BrdU assay (B). MTT, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide; BrdU+, bromodeoxyuridine positive. **p < 0.01; ***p < 0.001.
