Figure 2.
The correlation of HSP27 expression and DDP sensitivity ovarian cancer cell line. (A) Expression of HSP27 protein in human ovarian cancer cell lines as depicted by Western blot analysis. β-Actin serves as a loading control. Right panel is the respective relative expression of HSP27 protein in ovarian cancer cell lines. Data are mean ± SEM. β-Actin was a normalization control. (B) Cell viability was assayed after treatment with increasing concentrations of cisplatin (cDDP) for 48 h by CCK-8. Data are shown as the mean ± 95% confidence interval from three independent experiments. (C) The IC50 analysis in these six ovarian cancer cell lines showed an inverse trend between cisplatin sensitivity and HSP27 expression. Data are means of three separated experiments ± SD.