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Knockdown of CTHRC1 suppresses the activation of Wnt/β-catenin signaling in RCC cells. Caki-1 cells were transfected with si-CTHRC1 or vector for 24 h. (A) Western blot analysis of β-catenin, c-Myc, and cyclin D1 on the depletion of CTHRC1 in Caki-1 cells. GAPDH was used as a loading control. (B) Quantification analysis was performed using Gel-Pro Analyzer version 4.0 software. All values shown are mean ± SD of three independent experiments. *p < 0.05 versus vector group.
