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Effects of BMP-7 on TGF-β1-suppressed Smad signaling. NRK-52E cells were treated with TGF-β1 (5 ng/ml), BMP-7 (100 ng/ml), BMP-7 (100 ng/ml) plus TGF-β1 (5 ng/ml) for 24 h, and Western blot analysis was used to measure the protein levels of pSmad1, pSmad5, and pSmad8. Blots were incubated with pSmad1, pSmad5, and pSmad8 antibodies.
