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. 2016 Mar 25;23(4):147–154. doi: 10.3727/096504016X14519157902645

Figure 4.

Figure 4

Effects of miRNA-21 on TGF-β-induced FN secretion, apoptosis, and Smad signaling. (A) NRK-52E cells were transfected with miRNA-21 mimic or siRNA for 24 h, and the efficiency of overexpression and knockdown was measured by RT-PCR method. Data are expressed as mean ± SD of three independent experiments in triplicate. (B) Cells were treated with TGF-β1 (5 ng/ml), miRNA-21 mimic plus TGF-β1 (5 ng/ml), miRNA-21 siRNA plus TGF-β1 (5 ng/ml) for 8 h. Expression of FAS, FASL, and FN in cell culture was analyzed using ELISA method. Data are expressed as mean ± SD of three independent experiments in triplicate. (C) NRK-52E cells were treated with miRNA-21 siRNA or miRNA-21 mimic for 48 h, the protein levels of pSmad1, pSmad5, and pSmad8. Blots were incubated with pSmad1, pSmad5 and pSmad8 antibodies were measured using Western blot analysis.