Table 1.
Differentially expressed proteins after TBI by iTRAQ
| Accession | Description | Coverage (%) | MW (kDa) | Average | p-value | FC | |
|---|---|---|---|---|---|---|---|
| Sham | TBI | ||||||
| D3ZVU4 | Ribokinase (Rbks) | 11 | 34 | 121.2 | 78.8 | 0.00070 | 0.65 |
| P30337 | N-chimaerin (Chn1) | 4 | 38.2 | 120.4 | 79.7 | 0.0057 | 0.66 |
| B1PLB1 | CD34 antigen (Cd34) | 11 | 41.2 | 115.1 | 84.9 | 0.00030 | 0.74 |
| Q6XFR6 | Glycophorin-C (Gypc) | 27 | 10.4 | 113.0 | 87.0 | 0.0014 | 0.77 |
| P08050 | Gap junction alpha-1 protein (Gja1) | 17 | 43 | 113.0 | 87.0 | 0.00010 | 0.77 |
| P15384 | Potassium voltage-gated channel subfamily A member 3 (Kcna3) | 8 | 58.4 | 111.3 | 88.7 | 0.042 | 0.80 |
| P49655 | ATP-sensitive inward rectifier potassium channel 10 (Kcnj10) | 4 | 42.5 | 111.1 | 88.9 | 0.012 | 0.80 |
| Q6P7A9 | Lysosomal alpha-glucosidase (Gaa) | 4 | 106.1 | 110.9 | 89.0 | 0.00080 | 0.80 |
| D3Z900 | Mitochondrial amidoxime reducing component 2 (Marc2) | 16 | 38.2 | 110.6 | 89.4 | < 0.0001 | 0.81 |
| D4A871 | Leiomodin 3 (Lmod3) | 2 | 66 | 110.5 | 89.5 | 0.013 | 0.81 |
| P06685 | Sodium/potassium-transporting ATPase subunit alpha-1 (Atp1a1) | 46 | 113 | 110.3 | 89.7 | < 0.0001 | 0.81 |
| D3ZSW9 | RUN and FYVE domain-containing 2 (LOC100910755) | 2 | 69.9 | 110.3 | 89.8 | 0.013 | 0.81 |
| P29101 | Synaptotagmin-2 (Syt2) | 31 | 47.2 | 110.1 | 89.9 | < 0.0001 | 0.82 |
| P11730 | Calcium/calmodulin-dependent protein kinase type II subunit gamma (Camk2g) | 28 | 59 | 109.9 | 90.1 | 0.00020 | 0.82 |
| B4F7C1 | G protein-coupled receptor 37-like 1, isoform CRA_a (Gpr37l1) | 2 | 52.8 | 109.8 | 90.2 | 0.0060 | 0.82 |
| D4A617 | Ectonucleoside triphosphate diphosphohydrolase 1 (LOC103689954) | 3 | 52 | 109.8 | 90.2 | 0.0071 | 0.82 |
| Q8VHX0 | Voltage-dependent calcium channel gamma-3 subunit (Cacng3) | 10 | 35.5 | 109.6 | 90.4 | 0.0093 | 0.82 |
| P06686 | Sodium/potassium-transporting ATPase subunit alpha-2 (Atp1a2) | 45 | 112.1 | 109.4 | 90.7 | 0.00010 | 0.83 |
| A0A0G2K2B7 | WD repeat domain 20 (Wdr20) | 4 | 64.6 | 109.3 | 90.7 | 0.023 | 0.83 |
| G3V8R5 | Hormone-sensitive lipase (Lipe) | 1 | 116.8 | 109.3 | 90.8 | 0.044 | 0.83 |
| Q9Z2N6 | Calcium/calmodulin-dependent protein kinase II inhibitor 2 (Camk2n2) | 16 | 8.6 | 109.1 | 90.9 | 0.0095 | 0.83 |
| P20760 | Ig gamma-2A chain C region (Igg-2a) | 16 | 35.2 | 90.8 | 109.3 | 0.022 | 1.20 |
| Q9QYJ6 | cAMP and cAMP-inhibited cGMP 3′,5′-cyclic phosphodiesterase 10A (Pde10a) | 14 | 90.1 | 90.7 | 109.4 | 0.00040 | 1.21 |
| D3ZMR1 | Translocase of outer mitochondrial membrane 7 (Tomm7) | 11 | 6.2 | 90.6 | 109.4 | 0.0010 | 1.21 |
| P14173 | Aromatic-l-amino-acid decarboxylase (Ddc) | 11 | 54 | 90.6 | 109.4 | 0.0042 | 1.21 |
| Q99P74 | Ras-related protein Rab-27B (Rab27b) | 5 | 24.6 | 90.6 | 109.4 | 0.0062 | 1.21 |
| M0RDW3 | N-acetyltransferase 8 (GCN5-related) family member 4 (LOC102556148) | 3 | 25.6 | 90.4 | 109.6 | 0.0079 | 1.21 |
| D3ZM03 | Vacuolar ATPase assembly integral membrane protein VMA21 (LOC100912478) | 12 | 11.4 | 90.3 | 109.8 | 0.0044 | 1.22 |
| D3ZCC3 | Slc24a4 protein (Slc24a4) | 4 | 62.8 | 90.3 | 109.8 | 0.045 | 1.22 |
| P14942 | Glutathione S-transferase alpha-4 (Gsta4) | 8 | 25.5 | 90.1 | 110.0 | 0.043 | 1.22 |
| R9PXW6 | RAS guanyl releasing protein 2, isoform CRA_b (Rasgrp2) | 5 | 69.3 | 90.0 | 110.0 | 0.0025 | 1.22 |
| F1LXF1 | BCR, RhoGEF and GTPase-activating protein (Bcr) | 6 | 120 | 90.0 | 110.0 | 0.0088 | 1.22 |
| Q4QQT4 | Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform (Ppp2r1b) | 11 | 66 | 89.8 | 110.2 | 0.046 | 1.23 |
| Q5FVI0 | Arpp-21 protein (Arpp21) | 34 | 9.6 | 89.8 | 110.2 | 0.00010 | 1.23 |
| Q6JAM9 | Transmembrane protein 35A (Tmem35a) | 5 | 18.5 | 89.8 | 110.3 | 0.0054 | 1.23 |
| D3ZEY4 | Diacylglycerol kinase (Dgkq) | 3 | 102.5 | 89.7 | 110.3 | 0.0078 | 1.23 |
| A0A0G2JXN6 | Galectin (Lgals3) | 4 | 27.6 | 89.7 | 110.3 | 0.031 | 1.23 |
| D4A1H2 | Phosphatidylinositol-specific phospholipase C, X domain-containing 3 (Plcxd3) | 8 | 36.3 | 89.5 | 110.5 | 0.021 | 1.23 |
| F1LQY6 | N-terminal EF-hand calcium-binding protein 2 (Necab2) | 16 | 43.5 | 89.5 | 110.5 | 0.00020 | 1.23 |
| F1LST1 | Fibronectin (Fn1) | 4 | 262.6 | 89.5 | 110.5 | 0.0024 | 1.24 |
| D4A1J3 | Paralemmin 3 (Palm3) | 7 | 78.5 | 89.3 | 110.7 | 0.0020 | 1.24 |
| Q5M971 | Protein AF1q (Mllt11) | 8 | 10 | 89.2 | 110.8 | 0.012 | 1.24 |
| Q5M7T5 | Serine (Or cysteine) peptidase inhibitor, clade C (Antithrombin), member 1 (Serpinc1) | 16 | 52.2 | 89.1 | 110.9 | < 0.0001 | 1.24 |
| A0A0G2K896 | Similar to RIKEN cDNA 1300017J02 (RGD1310507) | 5 | 76.7 | 89.1 | 111.0 | 0.0014 | 1.25 |
| P34926 | Microtubule-associated protein 1A OS = Rattus norvegicus OX = 10,116 GN = Map1a PE = 1 SV = 1 | 32 | 299.3 | 89.0 | 111.1 | 0.00030 | 1.25 |
| A0A0G2K728 | Cadherin-4 (Cdh4) | 3 | 94.4 | 89.0 | 111.1 | 0.035 | 1.25 |
| P49805 | Regulator of G-protein signaling 9 (Rgs9) | 2 | 77.1 | 88.9 | 111.1 | 0.041 | 1.25 |
| P22057 | Prostaglandin-H2 d-isomerase (Ptgds) | 9 | 21.3 | 88.8 | 111.2 | 0.011 | 1.25 |
| Q8R490 | Cadherin 13 (Cdh13) | 13 | 78 | 88.7 | 111.3 | 0.00010 | 1.25 |
| A0A0G2K613 | Uncharacterized protein | 22 | 19.8 | 88.7 | 111.3 | < 0.0001 | 1.25 |
| F1M7Z2 | IQ and AAA domain-containing protein 1 (Iqca) | 1 | 99.3 | 88.5 | 111.6 | 0.0043 | 1.26 |
| Q7TSE9 | HCLS1-associated protein X-1 (Hax1) | 9 | 31.4 | 88.3 | 111.8 | 0.0070 | 1.27 |
| Q6J4I0 | Protein phosphatase 1 regulatory subunit 1B (Ppp1r1b) | 32 | 22.9 | 88.3 | 111.7 | 0.0016 | 1.27 |
| P19944 | 60S acidic ribosomal protein P1 (Rplp1) | 57 | 11.5 | 87.9 | 112.1 | 0.047 | 1.28 |
| A0A0G2KAY3 | Kininogen-1 (Kng1) | 12 | 44.2 | 87.4 | 112.6 | 0.00020 | 1.29 |
| A0A0G2JSR6 | Guanine nucleotide-binding protein subunit gamma (Gng7) | 54 | 7.7 | 87.3 | 112.7 | 0.00080 | 1.29 |
| P61459 | Pterin-4-alpha-carbinolamine dehydratase (Pcbd1) | 7 | 12 | 87.2 | 112.8 | 0.0020 | 1.29 |
| B4F7E8 | Niban-like protein 1 (Fam129b) | 3 | 84.7 | 87.0 | 113.1 | 0.00050 | 1.30 |
| F1M5V2 | GLI pathogenesis-related 2 (Glipr2) | 8 | 19 | 86.9 | 113.2 | 0.025 | 1.30 |
| P19939 | Apolipoprotein C-I (Apoc1) | 10 | 9.9 | 86.8 | 113.2 | 0.011 | 1.30 |
| Q5EBC0 | Inter alpha-trypsin inhibitor, heavy chain 4 (Itih4) | 9 | 103.7 | 86.7 | 113.3 | < 0.0001 | 1.31 |
| G3V8T7 | RCG51933, isoform CRA_a (Tdrkh) | 4 | 62 | 86.3 | 113.8 | 0.031 | 1.32 |
| P47727 | Carbonyl reductase [NADPH] 1 (Cbr1) | 39 | 30.6 | 86.1 | 113.9 | 0.0052 | 1.32 |
| A0A0G2JVX7 | Apolipoprotein A-IV (Apoa4) | 23 | 44.3 | 85.8 | 114.2 | 0.00020 | 1.33 |
| P04094 | Proenkephalin-A (Penk) | 12 | 30.9 | 85.7 | 114.3 | 0.0010 | 1.33 |
| A0A0G2JZB6 | Uncharacterized protein | 12 | 15.8 | 85.6 | 114.4 | 0.029 | 1.34 |
| P02764 | Alpha-1-acid glycoprotein (Orm1) | 11 | 23.6 | 85.4 | 114.6 | < 0.0001 | 1.34 |
| P62749 | Hippocalcin-like protein 1 (Hpcal1) | 26 | 22.3 | 85.4 | 114.6 | 0.00080 | 1.34 |
| P04177 | Tyrosine 3-monooxygenase (Th) | 28 | 55.9 | 85.3 | 114.7 | < 0.0001 | 1.34 |
| D3ZTN0 | Protein kinase, DNA-activated, catalytic polypeptide (Prkdc) | 0 | 471.8 | 85.2 | 114.8 | 0.010 | 1.35 |
| Q00495 | Macrophage colony-stimulating factor 1 receptor (Csf1r) | 1 | 109.2 | 85.2 | 114.8 | 0.031 | 1.35 |
| G3V7X2 | Scg2 protein (Scg2) | 4 | 66.6 | 85.1 | 114.9 | 0.0023 | 1.35 |
| Q9QYU4 | Ketimine reductase mu-crystallin (Crym) | 39 | 33.5 | 85.0 | 115.1 | < 0.0001 | 1.35 |
| P07150 | Annexin A1 (Anxa1) | 17 | 38.8 | 84.9 | 115.1 | 0.0034 | 1.35 |
| A0A0G2JZV3 | ATP/GTP-binding protein 1 (Agtpbp1) | 3 | 136.5 | 84.8 | 115.2 | 0.0061 | 1.36 |
| Q68FY4 | Group specific component (Gc) | 28 | 53.5 | 84.8 | 115.2 | < 0.0001 | 1.36 |
| Q64240 | Protein AMBP (Ambp) | 8 | 38.8 | 84.8 | 115.2 | 0.00020 | 1.36 |
| A0A0G2K3G0 | Histidine-rich glycoprotein (Hrg) | 4 | 60.3 | 84.7 | 115.3 | 0.0031 | 1.36 |
| P08934 | Kininogen-1 (Kng1) | 5 | 70.9 | 84.6 | 115.4 | 0.028 | 1.37 |
| Q9QX79 | Fetuin-B (Fetub) | 15 | 41.5 | 84.4 | 115.6 | 0.00010 | 1.37 |
| F1M609 | Acyl-coenzyme A oxidase (Acox1) | 3 | 66 | 84.3 | 115.7 | 0.0086 | 1.37 |
| A0A0G2K9I6 | Ceruloplasmin (Cp) | 10 | 123.6 | 83.8 | 116.2 | < 0.0001 | 1.39 |
| D3ZY96 | Neutrophilic granule protein (Ngp) | 20 | 19.4 | 83.8 | 116.2 | 0.0052 | 1.39 |
| Q07936 | Annexin A2 (Anxa2) | 12 | 38.7 | 83.8 | 116.2 | < 0.0001 | 1.39 |
| D3ZGF1 | CD44 antigen (Cd44) | 7 | 52.8 | 83.7 | 116.3 | 0.0001 | 1.39 |
| G3V6X7 | ProSAAS (LOC108348172) | 13 | 27.5 | 83.7 | 116.4 | 0.0021 | 1.39 |
| M0R3N4 | Vesicle amine transport 1-like (Vat1l) | 22 | 41.6 | 83.6 | 116.4 | < 0.0001 | 1.39 |
| Q5M878 | Serum amyloid A protein (Saa4) | 15 | 15 | 83.5 | 116.5 | 0.00010 | 1.39 |
| Q5M818 | 39S ribosomal protein L16, mitochondrial (Mrpl16) | 6 | 28.9 | 83.4 | 116.6 | 0.031 | 1.40 |
| A0A0G2JWX4 | Keratin, type II cytoskeletal 2 epidermal (Krt2) | 6 | 69.1 | 83.4 | 116.6 | < 0.0001 | 1.40 |
| Q63433 | Serine/threonine-protein kinase N1 (Pkn1) | 1 | 104.4 | 83.2 | 116.8 | 0.0027 | 1.40 |
| A0A0G2K526 | Guanine nucleotide-binding protein G(olf) subunit alpha (Gnal) | 9 | 51.3 | 83.0 | 117.0 | 0.00010 | 1.41 |
| P97738 | Neuronal pentraxin-2 (Nptx2) | 18 | 47.4 | 82.8 | 117.2 | 0.00010 | 1.42 |
| P20761 | Ig gamma-2B chain C region (Igh-1a) | 7 | 36.5 | 82.2 | 117.8 | < 0.0001 | 1.43 |
| A0A0G2JYK0 | Serine protease inhibitor A3N (LOC299282) | 27 | 67.9 | 81.8 | 118.2 | < 0.0001 | 1.44 |
| Q63910 | Alpha globin (Hba-a3) | 15 | 15.5 | 81.8 | 118.3 | 0.0056 | 1.45 |
| P47728 | Calretinin (Calb2) | 15 | 31.4 | 81.2 | 118.9 | < 0.0001 | 1.46 |
| P01355 | Cholecystokinin (Cck) | 9 | 12.8 | 80.9 | 119.1 | 0.0029 | 1.47 |
| D3ZQN7 | Laminin subunit beta 1 (Lamb1) | 1 | 197.3 | 80.8 | 119.2 | 0.038 | 1.48 |
| D4AA52 | Murinoglobulin-1 (LOC297568) | 13 | 163.6 | 80.7 | 119.3 | < 0.0001 | 1.48 |
| P01048 | T-kininogen 1 (Map1) | 6 | 47.7 | 80.7 | 119.3 | 0.012 | 1.48 |
| Q4FZU2 | Keratin, type II cytoskeletal 6A (Krt6a) | 7 | 59.2 | 80.4 | 119.7 | 0.017 | 1.49 |
| P06866 | Haptoglobin (Hp) | 19 | 38.5 | 80.3 | 119.7 | < 0.0001 | 1.49 |
| Q5I0M1 | Apolipoprotein H (Apoh) | 11 | 38.4 | 80.3 | 119.8 | 0.011 | 1.49 |
| Q6IFW6 | Keratin, type I cytoskeletal 10 (Krt10) | 13 | 56.5 | 80.2 | 119.8 | < 0.0001 | 1.49 |
| A0A0G2QC06 | Serotransferrin (Tf) | 24 | 107.3 | 80.1 | 120.0 | < 0.0001 | 1.50 |
| P02767 | Transthyretin (Ttr) | 20 | 15.7 | 79.8 | 120.2 | 0.00010 | 1.51 |
| F7FHF3 | Serpin family F member 2 (Serpinf2) | 5 | 62.3 | 79.6 | 120.5 | 0.00010 | 1.51 |
| D3ZFH5 | Inter-alpha-trypsin inhibitor heavy chain 2 (Itih2) | 4 | 92.3 | 79.4 | 120.6 | < 0.0001 | 1.52 |
| Q68G38 | Torsin-1A (Tor1a) | 3 | 37.9 | 79.3 | 120.7 | 0.034 | 1.52 |
| A0A0G2JUX5 | Transcriptional activator protein Pur-beta (Purb) | 24 | 33.5 | 79.3 | 120.7 | < 0.0001 | 1.52 |
| P20156 | Neurosecretory protein VGF (Vgf) | 21 | 68.1 | 78.7 | 121.3 | 0.026 | 1.54 |
| M0RBF1 | Complement C3 (C3) | 21 | 186.2 | 78.6 | 121.5 | < 0.0001 | 1.55 |
| P00697 | Lysozyme C-1 (Lyz1) | 16 | 16.7 | 77.4 | 122.6 | 0.00040 | 1.58 |
| A0A0G2JZ73 | Alpha-1-antiproteinase (Serpina1) | 39 | 46.6 | 77.4 | 122.6 | < 0.0001 | 1.59 |
| Q62930 | Complement component C9 (C9) | 2 | 62.2 | 77.3 | 122.8 | 0.00010 | 1.59 |
| Q6P6Q2 | Keratin, type II cytoskeletal 5 (Krt5) | 15 | 61.8 | 77.1 | 122.9 | < 0.0001 | 1.59 |
| G3V9R9 | Afamin (Afm) | 15 | 69.2 | 76.9 | 123.2 | < 0.0001 | 1.60 |
| P24090 | Alpha-2-HS-glycoprotein (Ahsg) | 26 | 38 | 76.6 | 123.4 | < 0.0001 | 1.61 |
| P20059 | Hemopexin (Hpx) | 23 | 51.3 | 76.5 | 123.6 | < 0.0001 | 1.62 |
| A0A0G2JSK1 | RCG20603 (Serpina3c) | 28 | 46.4 | 76.3 | 123.7 | < 0.0001 | 1.62 |
| A0A0H2UHJ1 | Protein S100-A9 (S100a9) | 11 | 15.2 | 76.0 | 124.0 | < 0.0001 | 1.63 |
| Q6IMF3 | Keratin, type II cytoskeletal 1 (Krt1) | 8 | 64.8 | 75.7 | 124.3 | < 0.0001 | 1.64 |
| F7F8G2 | Bromodomain testis-specific protein (Brdt) | 1 | 106.7 | 75.6 | 124.4 | < 0.0001 | 1.65 |
| P12346 | Serotransferrin (Tf) | 31 | 76.3 | 75.3 | 124.7 | 0.00010 | 1.66 |
| D3ZJF8 | Fc fragment of IgG-binding protein (Fcgbp) | 0 | 275 | 75.2 | 124.9 | < 0.0001 | 1.66 |
| A0A0H2UHI5 | Serine protease inhibitor A3N (Serpina3n) | 24 | 45.5 | 74.4 | 125.6 | < 0.0001 | 1.69 |
| Q63041 | Alpha-1-macroglobulin (A1m) | 10 | 167 | 74.4 | 125.7 | < 0.0001 | 1.69 |
| P10959 | Carboxylesterase 1C (Ces1c) | 11 | 60.1 | 74.2 | 125.9 | < 0.0001 | 1.70 |
| Q03626 | Murinoglobulin-1 (Mug1) | 13 | 165.2 | 73.7 | 126.3 | < 0.0001 | 1.71 |
| Q5BJY9 | Keratin, type I cytoskeletal 18 (Krt18) | 6 | 47.7 | 73.6 | 126.4 | 0.00040 | 1.72 |
| A0A0H2UI36 | Uncharacterized protein | 14 | 17 | 71.9 | 128.2 | < 0.0001 | 1.78 |
| P50115 | Protein S100-A8 (S100a8) | 22 | 10.2 | 71.6 | 128.4 | < 0.0001 | 1.79 |
| P42930 | Heat shock protein beta-1 (Hspb1) | 41 | 22.9 | 71.0 | 129.0 | < 0.0001 | 1.82 |
| Q6IFV1 | Keratin, type I cytoskeletal 14 (Krt14) | 15 | 52.7 | 70.8 | 129.2 | < 0.0001 | 1.82 |
| P04639 | Apolipoprotein A-I (Apoa1) | 41 | 30 | 70.8 | 129.2 | < 0.0001 | 1.83 |
| P02770 | Serum albumin (Alb) | 64 | 68.7 | 69.9 | 130.2 | < 0.0001 | 1.86 |
| Q62669 | Globin a1 (LOC103694855) | 46 | 16 | 68.8 | 131.2 | < 0.0001 | 1.91 |
| B0BNN3 | Carbonic anhydrase 1 (Ca1) | 8 | 28.3 | 67.7 | 132.3 | 0.00010 | 1.95 |
| M0RCX0 | Pterin-4 alpha-carbinolamine dehydratase 2 (Pcbd2) | 22 | 14.1 | 66.4 | 133.6 | 0.039 | 2.01 |
| P02091 | Hemoglobin subunit beta-1 (Hbb) | 82 | 16 | 64.3 | 135.7 | < 0.0001 | 2.11 |
| Q6IFU7 | Keratin, type I cytoskeletal 42 (Krt42) | 13 | 50.2 | 62.0 | 138.0 | < 0.0001 | 2.22 |
| P11517 | Hemoglobin subunit beta-2 (Hbb2) | 78 | 16 | 59.3 | 140.8 | < 0.0001 | 2.38 |
| P01946 | Hemoglobin subunit alpha-1/2 (Hba1) | 63 | 15.3 | 54.9 | 145.1 | < 0.0001 | 2.64 |
| A0A0G2JSH5 | Serum albumin (Alb) | 65 | 68.7 | 47.6 | 152.4 | 0.00010 | 3.21 |
| F1LRV4 | Heat shock 70 kDa protein 4 (Hspa4) | 41 | 94 | 20.9 | 179.1 | < 0.0001 | 8.56 |
MW molecular weight, TBI traumatic brain injury, FC fold change