Table 2.

Antibodies.

Target	Species/isotype	Manufacturer, clone (product number)	Fluor.a	App.b	Dilution	Blocking bufferd	Incubation bufferd
Primary antibodies
p75-NGFR	Mouse IgG1	Advanced Targeting Systems, ME20.4 (AB-N07)	U	FC	0.2 μL / 106 cells	N/A	N/A
HNK-1	Mouse IgM	Sigma, VC1.1 (C6680)	U	FC	0.2 μL / 106 cells	N/A	N/A
PDGFRβ	Mouse IgG2a	BD Biosciences, 28D4 (558820)	U	FC	1.25 μL / 106 cells	N/A	N/A
PDGFRβ	Rabbit IgG	Cell Signaling Technology, 28E1 (3169)	U	ICC	1:100	5% GS + 0.4% TX-100	0.4% TX-100
NG2	Mouse IgG2a	Millipore, 9.2.27 (MAB2029)	U	FC	2 μL / 106 cells	N/A	N/A
				ICC	1:100	5% GS + 0.4% TX-100	0.4% TX-100
SM22⍺	Rabbit polyclonal	Abcam, (ab14106)	U	ICC	1:1000	3% BSA + 0.1% TX-100	3% BSA
Calponin	Mouse IgG1	Sigma, hCP (C2678)	U	ICC	1:15000	3% BSA + 0.1% TX-100	3% BSA
⍺SMA	Mouse IgG2a	Lab Vision, 1A4 (MS-113-P)	U	ICC	1:100	5% milk + 0.1% TX-100	5% milk
CD31	Rabbit polyclonal	Lab Vision (RB-10333-P)	U	ICC	1:25	5% GS + 0.4% TX-100	0.4% TX-100
Isotype control	Mouse IgG1	BD Biosciences, MOPC-21 (554121)	U	FC	Note (c)	N/A	N/A
Isotype control	Mouse IgM	BD Biosciences, G155–228 (553472)	U	FC	Note (c)	N/A	N/A
Isotype control	Mouse IgG2a	BD Biosciences, G155–178	U	FC	Note (c)	N/A	N/A
Secondary antibodies
Mouse IgG1	Goat polyclonal	Invitrogen, (A-21240)	AF647	FC	1:500	N/A	N/A
Mouse IgM	Goat polyclonal	Invitrogen, (A-21042)	AF488	FC	1:500	N/A	N/A
Mouse IgG	Goat polyclonal	Invitrogen, (A-11002)	AF488	FC	1:500	N/A	N/A
				ICC	1:200	N/A	Note (e)
Rabbit IgG	Goat polyclonal	Invitrogen, (A-21428)	AF555	ICC	1:200	N/A	Note (e)
Rabbit IgG	Goat polyclonal	Invitrogen, (A-21245)	AF647	ICC	1:200	N/A	Note (e)

^aFluorophore: U, unconjugated; AF, Alexa Fluor

^bApplication: FC, Flow cytometry; ICC, immunocytochemistry

^cUse isotype control antibodies at the same concentration as the corresponding primary antibodies

^dSee recipe; N/A, not applicable (for FC or secondary antibodies for ICC)

^eUse incubation buffer for the corresponding primary antibody