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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Curr Protoc. 2021 Jan;1(1):e14. doi: 10.1002/cpz1.14

Figure 1. Outline of the protocols for N-terminal labeling with NHS ester and binding affinity measurement by MST.

Figure 1

Basic Protocol 1 describes how to conduct the NHS ester labeling reaction. Basic Protocol 2 describes how to purify the NHS ester labeling reaction and get the labeled protein ready for the MST measurement. Basic Protocol 3 describes the procedure to conduct the binding assay on an MST instrument.