Table 2.

Calculations of ΔΔG between wild-type and mutant Orf3a. The top rank mutations are listed in the table. DynaMut webserver was used to calculate the predicted ΔΔG. The negative values indicate the destabilization of protein upon mutation.

S. No	Wild type residue	Residue position	Mutant residue	ΔΔG DynaMut (kcal/mol)
1	G	49	V	1.74
2	V	88	L	1.507
3	V	90	F	1.512
4	V	112	F	1.30
5	R	126	S	−2.024
6	C	148	S	−1.741
7	I	158	V	−1.583
8	Y	160	H	−1.527
9	S	171	L	1.619
10	D	210	Y	1.442
11	G	224	V	−1.528
12	G	224	C	−1.717