Table 3.
Stability of VC in plasma and precipitant under various storage conditions (n = 6).
| Matrix | Storage conditions | Time | Concentration (mg/L) |
Precision | Accuracy | |
|---|---|---|---|---|---|---|
| Spiked | Measured | (%) | (%) | |||
| Plasma | Room temperature (RT) | 0.75 h | 1.5 | 1.32 | 3.67 | 88.17 |
| 40 | 44.13 | 3.59 | 110.3 | |||
| 1.5 h | 1.5 | 1.13 | 6.15 | 75.17 | ||
| 40 | 38.9 | 5.34 | 97.31 | |||
| 3 h | 1.5 | 1.02 | 10.04 | 67.82 | ||
| 40 | 32.63 | 6.95 | 81.6 | |||
| 4°C | 2 h | 1.5 | 1.32 | 8.09 | 88.1 | |
| 40 | 40.53 | 5.41 | 101.31 | |||
| 4 h | 1.5 | 1.2 | 5.85 | 79.67 | ||
| 40 | 36.78 | 6.99 | 91.92 | |||
| −40°C | 3 d | 1.5 | 1.37 | 1.92 | 91.33 | |
| 40 | 38.15 | 2.57 | 95.36 | |||
| 5 d | 1.5 | 1.49 | 6.88 | 99.69 | ||
| 40 | 41.82 | 7.62 | 104.62 | |||
| 8 d | 1.5 | 0.87 | 4.12 | 58.23 | ||
| 40 | 34.77 | 4.17 | 86.94 | |||
| Precipitant | RT | 2 h | 1.5 | 1.53 | 4.23 | 101.93 |
| 40 | 39.12 | 2.12 | 97.76 | |||
| 4 h | 1.5 | 1.44 | 3.46 | 95.74 | ||
| 40 | 38.08 | 2.16 | 95.23 | |||
| Long term (-40°C) | 3 d | 1.5 | 1.57 | 2.07 | 104.95 | |
| 40 | 42.85 | 7.49 | 107.18 | |||
| 5 d | 1.5 | 1.63 | 3.81 | 108.48 | ||
| 40 | 39.17 | 2.86 | 97.9 | |||
| Autosampler | Autosampler (4°C) | 24 h | 1.5 | 1.63 | 4.32 | 108.48 |
| 40 | 44.47 | 3.32 | 111.17 | |||
| Plasma | Three-freeze-thaw cycles | 1.5 | 1.35 | 3.23 | 89.91 | |
| 40 | 43.15 | 1.34 | 107.82 | |||