Fig. 7.

Depletion of macrophages and specific inhibition of Nrlp3 protects the liver from cholestasis‐induced liver injury. (A) FC analysis on liver‐isolated immune cells from GF, GF + WT, clodronate/GF+WT, and MCC950/GF + WT mice all treated with ANIT for 48 hours to induce cholestasis and further (B) quantification showing reduced macrophage infiltration in clodronate‐ and MCC950‐treated mice. (C) Western blotting analysis of whole‐liver lysates showing whole and cleaved caspase 1 and whole and cleaved IL1B. (D) qPCR analysis on liver extracts showing decreased inflammation, (E) reduced transaminase levels, and AP and (F) improved liver parenchyma status in H&E staining. n = 4‐8 animals per treatment group were analyzed. Values are mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001 (ANIT/GF + WT vs. clodronate/GF + WT and MCC950//GF + WT). Abbreviations: ALT, alanine aminotransferase; AST, aspartate aminotransferase; FC, flow cytometry; H&E, hematoxylin and eosin; TLR2, Toll‐like receptor 2.