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. 2020 Nov 28;26(1):31–39. doi: 10.1111/gtc.12818

FIGURE 4.

FIGURE 4

Injection of wild‐type hps4.L mRNA, but not mutant hps4.L mRNA, into mutant embryos rescued the albino phenotype. (a–c) Stage 33/34 embryos; (d–h) stage 40 embryos. Uninjected control embryos of the wild type (a, d) and the mutant (b, e) were compared with mutant embryos injected with either mutant hps4.L mRNA (f) or wild‐type hps4.L mRNA (c, g, h). Although melanin did not appear in mutant control embryos at stages 33/34 (b) and 40 (e), mutant embryos injected with wild‐type hps4.L mRNA expressed melanin in the eyes (black arrows) and melanophores (arrowheads) at stages 33/34 (c) and 40 (g, h). The expression of melanin in mutant embryos injected with wild‐type hps4.L mRNA (c, g) was similar to that of wild‐type controls (a, d). Embryos injected with mutant hps4.L mRNA did not express melanin at stage 40 (f), and were similar to mutant controls (e). Dorsal view of the head and trunk regions of mutant embryo injected with wild‐type hps4.L mRNA shows that melanin appeared in the eye of the injected area (h, black arrow), but not in the eye of the uninjected area (h, white arrow)