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. 2020 Nov 28;253(2):209–224. doi: 10.1002/path.5574

Figure 1.

Figure 1

miR‐200c‐3p inhibition prevents EndoMT. (A) Expression levels of the miR‐200 family during EndoMT. HUVECs were incubated with 5 ng/ml TGFβ1 and 5 ng/ml TNFα for the indicated times to induce EndoMT. (B, C) Inhibition of miR‐200c‐3p prevents EndoMT. HUVECs were transfected with reagent control only (Ctrl), a scrambled negative control miRNA inhibitor (SCR inhibitor) or miR‐200c‐3p inhibitor (miR‐200c inhibitor), respectively. Transfected cells were incubated with 5 ng/ml TGFβ1/TNFα for 4 days. Total RNAs and proteins were harvested and subjected to RT‐qPCR (B) and western blot (C) analysis, respectively. Left panel in C: representative images; right panel: quantitative results of five independent experiments. The data presented here are mean ± SEM of five (n = 5) independent experiments. *p < 0.05, **p < 0.01, or ***p < 0.001 (versus D0 or Ctrl/SCR; one‐way ANOVA with a Tukey's post hoc test).