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Gating scheme of AIM enrichment of polyclonal ZIKV-reactive CD4 T cell lines. Left four panels illustrate sequential gating scheme of single cells followed by live CD3 T cells and then CD4+CD8- cells. At right, a representative donor shows in the upper panel low CD137 expression 18 hours after stimulation with negative control Vero cell preparation, and in contrast specific activation of rare CD4 T cell after stimulation with cell-associated, UV-treated ZIKV cell lysate.
