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. Author manuscript; available in PMC: 2021 Jan 27.
Published in final edited form as: Immunohorizons. 2020 Aug 4;4(8):444–453. doi: 10.4049/immunohorizons.2000068

Table 3.

T cell epitopes in ZIKV detected using bulk ZIKV viral lysate-reactive CD4 T cell lines.

donor protein and AA sequence HLA loci1 HLA restricting allele(s)2
4 ENV [301–320] KGVSYSLCTAAFTFTKIPAE DP HLA-DPB1*04:01
4 NS1 [91–110] GSVKNPMWRGPQRLPVPVNE DP HLA-DPB1*04:01
4 NS1 [101–120] PQRLPVPVNELPHGWKAWGK DP HLA-DPB1*04:01
4 NS2B[36–55] GLLIVSYVVSGKSVDMYIER DR HLA-DRB1*04:11
10 ancC [10–29] GFRIVNMLKRGVARVSPFGG HLA-DPB1*02:01
10 ancC [82–101] KKFKKDLAAMLRIINARKEK
10 ENV [101–120] WGNGCGLFGKGSLVTCAKFA HLA-DQB1*03:01
10 ENV [111–130] GSLVTCAKFACSKKMTGKSI HLA-DRB3*02:02
10 ENV [131–150] QPENLEYRIMLSVHGSQHSG HLA-DPB1*02:01
10 NS1 [141–160] KECPLKHRAWNSFLVEDHGF
12 NS1 [31–50] RYKYHPDSPRRLAAAVKQAW DR
12 NS1 [91–110] GSVKNPMWRGPQRLPVPVNE DP
17 ancC [10–29] GFRIVNMLKRGVARVSPFGG DR HLA-DRB1*13:01
17 ancC [37–56] LLLGHGPIRMVLAILAFLRF DR
17 ancC [46–65] MVLAILAFLRFTAIKPSLGL DR/DP HLA-DRB1*13:01
17 ENV [61–80] YEASISDMASDSRCPTQGEA DQ HLA-DQB1*03:02
17 ENV [391–410] VGEKKITHHWHRSGSTIGKA DR HLA-DRB1*04:07
17 NS1 [81–100] ENGVQLTVVVGSVKNPMWRG DQ HLA-DRB1*13:01, HLA-DQB1*06:02
17 NS1 [151–170] NSFLVEDHGFGVFHTSVWLK DP HLA-DPB1*04:02, HLA-DQB1*03:02
17 NS1 [161–180] GVFHTSVWLKVREDYSLECD DP HLA-DPB1*04:02, HLA-DQB1*03:02
17 NS1 [201–220] WIESEKNDTWRLKRAHLIEM HLA-DPB1*04:02
17 NS5 [221–240] WVSGAKSNTIKSVSTTSQLL DR HLA-DRB1*04:07, HLA-DRB1*13:01
17 PreM [64–83] DVDCWCNTTSTWVVYGTCHH DQ HLA-DQB1*06:02
17 PreM [73–92] STWVVYGTCHHKKGEARRSR DQ HLA-DQB1*06:02
24 ENV [131–150] QPENLEYRIMLSVHGSQHSG DR HLA-DRB1*01:01
24 NS4A [73–92] MRNKGIGKMGFGMVTLGASA DQ HLA-DQB1*06:02
24 NS4B [13–32] LSHLMGRREEGATIGFSMDI DQ HLA-DQB1*06:02
24 NS4B [22–41] EGATIGFSMDIDLRPASAWA DQ HLA-DQB1*06:02
24 NS4B [31–50] DIDLRPASAWAIYAALTTFI
24 NS5 [41–60] RRALKDGVATGGHAVSRGSA DR HLA-DRB1*01:01, HLA-DRB5*01:01
1

Specificity of HLA locus-specific mAb or mAbs causing substantial inhibition of proliferative responses by bulk ZIKV-reactive CD4 T cell lines in 3H thymidine incorporation assays. In some instances, inhibition was not noted with any anti-HLA mAb while in others, more than one mAb led to inhibition.

2

HLA loci expressed by aAPC leading to specific secretion of IFNγ by bulk ZIKV-reactive CD4 T cell lines. In some instances, reactive aAPC were not detected.