Fig 2.
Extended bacterial artificial chromosome (BAC) clone transgenes that contain mouse Cyp27b1 and Cyp24a1 genetic loci recapitulate the hormonal regulation by exogenous PTH, FGF23, and 1,25(OH)2D3 regulation seen for endogenous genes in mice. (A) Schematic depiction of mouse transgene structures. (B) Hormonal regulation. Transgenic mice were prepared and selected as recently reported.( 22 ) Animals were injected with PTH (230 ng/g body weight [BW]; blue), FGF23 (50 ng/g BW; green), or 1,25(OH)2D3 (10 ng/g BW; black) and tissues harvested at 1, 3, and 6 hours, respectively. Transgene‐derived Cyp27b1 and Cyp24a1 transcripts were quantitated using probes that required the presence of the internal ribosome entry‐site module incorporated into the BAC clones. Data are derived from four to six mice per group, and presented as the means ± SEM, *p < 0.05.