Figure 4.

Neutralization potency of human serum against each VSV-SARS-CoV-2 mutant

(A) Neutralization potency of four human sera against VSV-SARS-CoV-2 mutants. IC₅₀ values were calculated from three independent experiments. Neutralization potency is represented as a rainbow color map from red (most potent with low IC₅₀) to violet (less potent with high IC₅₀). LOD indicates limit of detection (1:80).

(B) Representative neutralization curves of wild-type, S477N, and E484A mutant with four different human sera. Error bars represent SEM. Data are representative of three independent experiments.

(C) Neutralization potency of additional 16 human sera against VSV-SARS-CoV-2 mutants. IC₅₀ values were calculated from one independent experiment each. Neutralization potency is represented as a rainbow color map from red (most potent with low IC₅₀) to violet (less potent with high IC₅₀). Neutralization curves are provided in Figure S5.

(D) Serum samples from 18 individuals were collected at different time points post-onset of COVID-19 symptoms and screened using two ELISA assays (Euroimmun or Epitope). The serum identifier numbers in the first column correspond to those of Figures 4 and S5. IgG index values were calculated by dividing the optical density (O.D.) of the serum sample by a reference O.D. control, and ratios were interpreted using the following criteria as recommended by the manufacturer: negative (−) < 0.8, indeterminate (+/−) 0.8–1.1, and positive (+) ≥ 1.1.