Figure 5.
Wnt/β‐catenin signalling pathway took part in apoptosis of H2O2‐induced myocardial cells. A, Expressions of Wnt3a, Wnt5a and β‐catenin in H9c2 cells treated after H2O2 were measured with Western blot, P < .05. B, Levels of Wnt3a, Wnt5a and β‐catenin with transfected circ‐ITCH and miR‐17‐5p and after NCB‐0846 treatment were measured using Western blot, P < .05. C, Cell viabilities after NCB‐0846 treatment were analysed with CCK‐8, P < .05. D, ATP concentrations with NCB‐0846 added were analysed through ATP Assay Kit, P < .05. E, Apoptosis rate were evaluated using flow cytometry, P < .05. F, Western blot was performed for measuring expressions of caspase 3, p53 and PARP, P < .05. Student's t test and one‐way ANOVA were used