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. Author manuscript; available in PMC: 2021 Jan 27.
Published in final edited form as: Cell Rep Phys Sci. 2020 Dec 9;1(12):100270. doi: 10.1016/j.xcrp.2020.100270

Figure 2. Perimitochondrial ENS to Deliver Oligonucleotides, Nucleic Acids, and Plasmids into Mitochondria.

Figure 2.

(A) HeLa cells, incubated with Mito-FLAG and FITC-labeled oligonucleotides (100 nM, 24 h), display green fluorescence of FITC in mitochondria.

(B) Fluorescent images of mtDNA-depleted HeLa cells incubated with 1 mL pMAX-DEST plasmid (10 μg/mL, 24 h) mixed with or without Mito-FLAG.

(C) Gel electrophoresis analysis of pGLO plasmid in the pellet of Mito-FLAG after high-speed centrifuge.

(D) Fluorescent images of the HeLa cells incubated with pGLO and Mito-pGLO plasmid (5 μg/mL, 24 h, 0.2 wt% arabinose) in the presence of Mito-FLAG.

(E) Detection of pGLO plasmid in the mitochondria isolated from the HeLa cells treated by pGLO (5 μg/mL) and Mito-FLAG via PCR and DNA electrophoresis.

(F) Fluorescent images of the HeLa cells expressed pGLO (in mitochondria) before and after one passage. All Mito-FLAG concentrations are 200 μM.