Table 1.
Medium and high quality metagenome-assembled genomes (MAGs) affiliated to Methylococcales in Lake Lovojärvi, their completeness, contamination, size, corrected size (using completeness values), taxonomic cluster and grouping based on their vertical distribution pattern, as well as their genes encoding enzymes involved in methane oxidation, methanol oxidation, N2 fixation and hydroxylamine (NH2OH) detoxification. Medium and high quality are defined as completeness >50% and contamination <10%, and, as completeness >90% and contamination <5%, respectively. Four MAGs with the lowest contamination % were also given direct ENA accession numbers, which are also shown. The analysis for depth distribution grouping is presented in Fig. S5C, while the procedure for taxonomic classification is explained in detail in Supplementary Results and Discussion, Table S2 and Figs.S3, S4, S6 and S7. Full names of the enzymes are provided in the table footnotes.
| MAG (accession) | Compl. (%) | Contam. (%) | Size (corrected) (Mb) | Taxonomic cluster | Vertical distribution group | CH4 → CH3OH | CH3OH → CH2O | N2–fixat. | NH2OH detox. |
|---|---|---|---|---|---|---|---|---|---|
| MAG 1608 (ERS4666687) | 98.6 | 1.0 | 3.3 (3.4) | 1 (Methylobacter sp.) | 1A | pmoCAB | xoxF | ||
| MAG 2248 | 88.4 | 5.7 | 2.6 (3.0) | 3 (CABC2E06) | 1A | xoxF | |||
| MAG 2500 | 94.8 | 5.8 | 2.9 (3.1) | 1 (Methylobacter sp.) | 1A | mmoC | xoxF | nifH | haoAB |
| MAG 3581 (ERS4666738) | 65.2 | 1.8 | 2.8 (4.2) | 4 (Methylovulum/Methylosoma-like) | 1B | nifH | |||
| MAG 4043 (ERS4666746) | 93.7 | 2.0 | 2.8 (3.0) | 3 (CABC2E06) | 1B | pmoCAB | xoxF | nifH | |
| MAG 2066 | 89.5 | 6.8 | 3.2 (3.5) | 1 (Methylobacter sp.) | 2A | pmoC | |||
| MAG 2588 | 75.9 | 7.4 | 3.4 (4.4) | 2 (Lake Crenothrix) | 2A | pmoCA; smmoXYBZDC | xoxF | ||
| MAG 2715 | 88.9 | 5.4 | 2.4 (2.7) | 3 (CABC2E06) | 2A | pmoCAB | xoxF | ||
| MAG 1620 | 97.9 | 6.6 | 5.1 (5.2) | 1 (Methylobacter sp.) | 2B | pmoCAB | xoxF | nifH | |
| MAG 959 (ERS4666674) | 95.4 | 3.8 | 5.8 (6.1) | 5 (Ca. Methyloumidiphilus sp.) | 2B | pmoCAB (pxmABC) | xoxF | nifH | haoA; cytL |
pmoCAB/smmoXYBZDC = particulate/soluble methane monooxygenase, pxmABC = sequence-divergent particulate methane monooxygenase, xoxF = lanthanide-dependent methanol-dehydrogenase, nifH = nitrogenase iron protein, haoAB = hydroxylamine oxidoreductase and cytL = cytochrome P460