Fig. 3. Heme remodels REV-ERBβ LBD interaction with an NCoR RID construct composed of both ID motifs.

Representative ITC thermograms and fitted curves of apo (A) or heme-bound (B) REV-ERBβ LBD and NCoR RID constructs; data are representative of at least two experiments per condition (error bars, uncertainty in each injection calculated by the NITPIC algorithm). Calculated binding affinities are shown for the fitted datasets.