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. 2021 Jan 27;7(5):eabc6479. doi: 10.1126/sciadv.abc6479

Table 2. Thermodynamic parameters of NCoR RID binding to REV-ERBβ LBD.

	Apo LBD		⁺Heme LBD
	WT RID	ΔID2 RID	WT RID
K_D (nM)	Site 1: 12 (95% *CI: 1.4 to 56)	130 (95% CI: 100 to 180)	99 (95% CI: 46 to 460)
K_D (nM)	Site 2: 740 (95% CI: 380 to 1500)	130 (95% CI: 100 to 180)	99 (95% CI: 46 to 460)
ΔG (kcal/mol)	Site 1: −10.8 (95% CI: −12.1 to −9.9)	−11.1 (95% CI −11.5 to −10.8)	−9.6 (95% CI: −10.0 to −8.6)
ΔG (kcal/mol)	Site 2: −8.4 (95% CI: −8.8 to −8.0)	−11.1 (95% CI −11.5 to −10.8)	−9.6 (95% CI: −10.0 to −8.6)
ΔH (kcal/mol)	Site 1: −8.3 (95% CI: −9.1 to −7.7)	−9.4 (95% CI −9.6 to −9.2)	1.7 (95% CI: 1.4 to 2.0)
ΔH (kcal/mol)	Site 2: 3.2 (95% CI: 2.4 to 4.6)	−9.4 (95% CI −9.6 to −9.2)	1.7 (95% CI: 1.4 to 2.0)
^†TΔS (kcal/mol)	Site 1: 2.5 (95% CI: 2.2 to 3.0)	−1.8 (95% CI −1.9 to −1.6)	11.2 (95% CI: 10.7 to 11.4)
^†TΔS (kcal/mol)	Site 2: 12 (95% CI: 11 to13)	−1.8 (95% CI −1.9 to −1.6)	11.2 (95% CI: 10.7 to 11.4)

*CI calculated using SEDPHAT analysis software.

†Temperature (T), which was constant at 25°C, and entropy (ΔS) of binding.