Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Plasmodium falciparum) | KC5 | • 10.1128/mBio.02293–17 | Wt control | |
| Cell line (Plasmodium falciparum) | CLAG3-tv2 | This paper | C-terminal His10-FLAG-thrombin-TEV-HA-twinstrept-BC2 tag |
|
| Cell line (Plasmodium falciparum) | CLAG3-GFP | This paper | C-terminal His8-mGFP-FLAG-Twin Strep tag | |
| Cell line (Plasmodium falciparum) | CLAG3-tv1 | This paper | C-terminal 3xFLAG-3xHA-His8-Strept II tag | |
| Cell line (Plasmodium falciparum) | CLAG3-tv1+RhopH2-mV | This paper | CLAG3-tv1 with tandem RhopH2 C-terminal mVenus tag | |
| Cell line (Plasmodium falciparum) | 13F10 | • 10.1038/nature13574 | TMP-dependent HSP101 conditional knockdown | |
| Antibody | Anti-CLAG3 (mouse polyclonal) | • 10.7554/eLife.23485 | (1:1000) | |
| Sequence-based reagent | CLAG3 sgRNA | This paper | For CRISPR editing | 5′-TAAAAACACTAATAAGACCA-3′ |
| Recombinant DNA reagent | pUF1-Cas9 | • 10.1038/nbt.2925 | Cas9 expression | |
| Recombinant DNA reagent | pL6 | • 10.1038/nbt.2925 | sgRNA expression and homology cassette | |
| Recombinant DNA reagent | pL7- CLAG3-tv2 | This study | Modification of pL6 for parasite transfection | |
| Recombinant DNA reagent | pL7-CLAG3-GFP | This study | Modification of pL6 for parasite transfection | |
| Recombinant DNA reagent | pL7-CLAG3-tv1 | This study | Modification of pL6 for parasite transfection | |
| Chemical compound, drug | DSM1 | BEI Resources Repository | Cat# MRA-1161 | |
| Chemical compound, drug | WR99210 | David Jacobus | ||
| Commercial assay or kit | Anti-FLAG M2 affinity agarose resin | Sigma–Aldrich | Cat# A2220 | |
| Commercial assay or kit | 3xFLAG peptide | Sigma–Aldrich | Cat# F4799 | |
| Commercial assay or kit | Ni-NTA Agarose resin | Qiagen | Cat# 30210 | |
| Commercial assay or kit | Zeba microspin desalting columns, 40 kDa MWCO | Thermo Scientific | Cat# 87764 | |
| Other | Gold-coated quartz emitter | This study | Native mass MS study | |
| Commercial assay or kit | SYPRO Orange | Thermo Scientific | Cat# S6650 | Protein stability assay (1:5000) |
| Other | Carbon film grids | Electron Microscopy Sciences | Cat# CF200-Cu | |
| Other | Quantifoil Cu 300 mesh grids | Electron Microscopy Sciences | Cat# Q3310CR1.3 | |
| Other | 4–15% Mini-PROTEAN TGX gel | Bio-RAD | Cat# 4561086 | |
| Software, algorithm | Thermo Xcalibur Qual Browser | Thermo Scientific | versions 3.0.63 and 4.2.47 | |
| Software, algorithm | UniDec | • 10.1021/acs.analchem.5b00140; • 10.1007/s13361-018-1951-9 |
versions 3.2 and 4.1 | http://unidec.chem.ox.ac.uk/ |
| Software, algorithm | m/z | Proteometrics LLC | ||
| Software, algorithm | EPU | ThermoFischer | ||
| Software, algorithm | Latitude | Gatan Inc | ||
| Software, algorithm | RELION 2.0; RELION 3.0 | • 10.1016/j.jsb.2012.09.006 | https://www3.mrc-lmb.cam.ac.uk/relion | |
| Software, algorithm | MotionCor2 | • 10.1038/nmeth.4193 | https://emcore.ucsf.edu/ucsf-software | |
| Software, algorithm | Gctf | • 10.1016/j.jsb.2015.11.003 | https://www2.mrc-lmb.cam.ac.uk/research/locally-developed-software/zhang-software/ | |
| Software, algorithm | UCSF Chimera | • 10.1002/jcc.20084 | https://www.cgl.ucsf.edu/chimera/ | |
| Software, algorithm | Coot | • 10.1107/S0907444910007493 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ | |
| Software, algorithm | PHENIX assign_sequence | • 10.1107/S2059798319011471 | https://www.phenix-online.org/ | |
| Software, algorithm | PHENIX real space refine | • 10.1107/S2059798318006551 | https://www.phenix-online.org/documentation/reference/real_space_refine.html | |
| Software, algorithm | JPred | • 10.1093/nar/gkv332 | http://www.compbio.dundee.ac.uk/jpred/ | |
| Software, algorithm | elNémo server | • 10.1093/nar/gkh368 | http://www.sciences.univ-nantes.fr/elnemo/ | |
| Software, database | PlasmoDB | • 10.1093/nar/gkn814 | https://plasmodb.org/plasmo/ | |
| Software, algorithm | MAFFT server | • 10.1093/bib/bbx108 | https://mafft.cbrc.jp/alignment/server/ | |
| Software, algorithm | MEGA X | • 10.1093/molbev/msy096;10.1093/molbev/msz312 | https://www.megasoftware.net/ | |
| Software, algorithm | ConSurf server | • 10.1093/nar/gkw408 | https://consurf.tau.ac.il/ | |
| Software, algorithm | NCBI Protein BLAST | • 10.1093/nar/25.17.3389 | https://blast.ncbi.nlm.nih.gov/Blast.cgi | |
| Software, algorithm | Clustal Omega | • 10.1093/nar/gkz268 | https://www.ebi.ac.uk/Tools/msa/clustalo/ | |
| Software, algorithm | Pymol | Schrödinger, LLC | https://pymol.org/2/ | |
| Software, algorithm | Dali server | • 10.1093/bioinformatics/btz536 | http://ekhidna2.biocenter.helsinki.fi/dali/ | |
| Software, algorithm | ImageJ | • 10.1186/s12859-017-1934-z | https://imagej.nih.gov/ij/index.html | |
| Software, algorithm | SigmaPlot 10.0 | Systat | ||
| Software, algorithm | Prism 8.2 | GraphPad |