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. 2021 Jan 18;10:e59350. doi: 10.7554/eLife.59350

Figure 7. m138 is capable to restrict CD8+ T-cell activation by downregulating ICOSL on the surface of antigen-presenting cells (APCs).

Figure 7.

(A) C57BL/6-derived BMDCs were mock-infected or infected for 24 hr with MCMVwt or MCMVm138 mutants at an moi of 3 and analyzed by flow cytometry for surface expression of ICOSL and CD80 using specific mAbs against each of these molecules. Gray histograms represent the expression of mock-infected cells and colored histograms represent the expression of cells infected (positive for the MCMV m04 protein) with MCMVwt (yellow), MCMVΔm138 (pink), MCMVm138ΔIg2 (blue), or MCMVm138ΔIg2/3 (red). The isotype for each antibody was used as a negative control (black line histograms). Panels on the right represent the MFI of each sample minus the MFI of the control isotypes. Results are representative of two independent experiments (B) On the left, schematic representation of the in vitro antigen-presentation assay. BMDCs infected as indicated in A with MCMVwt (yellow), MCMVΔm138 (pink), MCMVm138ΔIg2 (blue), or MCMVm138ΔIg2/3 (red), or left uninfected (gray) were further co-cultured with naïve CD8+ T cells from Maxi mice. After 6 hr, IFN-γ production by CD8+ T cells was determined by flow cytometry. Results are representative of three independent experiments. Results are expressed as the mean +/-SEM of the percentage values obtained for samples in each group.