FIG. 5.

Suppression of cell cycle gene expression during intestinal remodeling in TR^−/− tadpoles. (A) Mapping of TRα-specific T3-regulated genes to cell cycle pathway (KEGG). Genes regulated by T3 for at least 2.0-fold, after 18 hours of T3 treatment of wild-type but not TRα^−/− tadpoles were mapped onto the KEGG pathway for the cell cycle. Pink boxes indicate upregulation, and blue boxes indicate downregulation. Note that most of regulated genes were upregulated by T3. (B) Bar graph representation of the expression of cell cycle genes from RNA-seq. Note that cdk1 (cdk1), cyclin A2 (ccna2), cyclin B1 (ccnb1), cyclin B2 (ccnb2), and cyclin B3 (ccnb3) were upregulated by T3 only in the wild-type intestine. The asterisk (*) indicates a significant difference between the TRα^−/− and wild type (FDR <0.05). FDR, false discovery rate; KEGG, Kyoto Encyclopedia of Genes and Genomes.