Table 2.
CC50 and EC50 of PLE in various cell lines and viruses.
| Cell line or virus strain | CC50 (mg/mL)a | EC50 (mg/mL)b | SIc |
|---|---|---|---|
| Vero E6 | 4.64 0.16 | ||
| Calu-3 | >5 | ||
| Huh7 | >1.25 | ||
| RD | 10.92 ± 0.47 | ||
| MDCK |
1.73 ± 0.39 |
||
| HCov-229E | >1.25 | – | |
| SARS-CoV-2 | 0.12 ± 0.06 | 40.65 | |
| EV-A71 | 0.04 ± 0.00 | 280 | |
| Influenza A/WSN/1933 | 1.09 ± 0.37 | 1.58 |
a
CC50: Drug concentration causing 50% cytotoxicity as determined by the MTT assay. Vero E6, Huh7, RD, and MDCK cells were incubated with PLE for 3 days, whereas Calu-3 cells for 2 days.
b
EC50: Concentration of compounds inhibiting 50% of the cytopathic effects caused by viral infection according to the MTT assay. EC50 is presented as mean ± standard deviation of two-three independent experiments. HCoV-229E and SARS-CoV-2 were assayed in Huh7 and Vero E6 cells, respectively.
c
Selectivity index (SI): CC50/EC50.