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. 2021 Jan 18;42(1):14–27. doi: 10.24272/j.issn.2095-8137.2020.186

Figure 2.

Figure 2

Knockdown Dmrt1 induced apoptosis and reduced proliferation of mGSCs

A: qRT-PCR detection of mRNA levels of inflammatory factors in testis-H1 and testis-shDmrt1. B: Western blotting detection of protein levels of inflammatory factors in testis-H1 and testis-shDmrt1. C: Protein levels were quantified using ImageJ software and normalized to GAPDH. D: Immunofluorescence detection of PCNA and TUNEL assay in testis-H1 and testis-shDmrt1. Scale bar: 100 μm. E: Western blotting detection of protein levels of apoptosis- and proliferation-related genes in testis-H1 and testis-shDmrt1. F: Protein levels were quantified using ImageJ software and normalized to GAPDH. *: P<0.05;**: P<0.01;***: P<0.001.