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. 2021 Jan 18;42(1):14–27. doi: 10.24272/j.issn.2095-8137.2020.186

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Editorial Office of Zoological Research, Kunming Institute of Zoology, Chinese Academy of Sciences

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Dmrt1 repressed apoptosis and promoted proliferation of mGSCs

A: Growth curve and population doubling time (PDT) of mGSCs and MSCs under treatment with different concentrations of LPS. B: Flow cytometry of cell cycle in mGSCs and MSCs under LPS treatment (0, 0.5, and 1 μg/mL). C: Flow cytometry of cell apoptosis in mGSCs and MSCs under LPS treatment (0, 0.5, and 1 μg/mL). D: Western blotting of cyclin-D1, PCNA, and caspase-3 in mGSCs under treatment with different concentrations of LPS. E: Protein levels were quantified using ImageJ software and normalized to GAPDH. F: Flow cytometry of cell cycle in LPS-mGSCs, LPS-mGSCs-H1, and LPS-mGSCs-shDmrt1. G: Western blotting of IL-6, PCNA, and cyclin-D1 in LPS-mGSCs-Dmrt1 and LPS-mGSCs-Plzf. H: Protein levels were quantified using ImageJ software and normalized to GAPDH. ^*: P<0.05;^**: P<0.01;^***: P<0.001.