FIGURE 4.

Hippocampal infusion of ARN-3236 induced notable antidepressant-like actions in the CUMS model of depression, as revealed by the FST (B), TST (C) and SPT (D). Schematic timeline of the experimental procedures is shown in (A). (E) Representative western blotting images and quantitative analyses show the effects of CUMS and ARN-3236 on hippocampal SIK2 and total CRTC1. (F) Representative western blotting images and quantitative analyses show the effects of CUMS and ARN-3236 on nuclear CRTC1 and cytoplasmic pCRTC1 in the hippocampus. All results were represented as means ± S.E.M (n = 10 for B–D, n = 5 for E–F); * p < 0.05, **p < 0.01. The comparisons were made by two-way ANOVA followed by Bonferroni's test.