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. 2021 Jan 14;11:615505. doi: 10.3389/fphar.2020.615505

FIGURE 5.

FIGURE 5

ABT-263 selectively induced HSFs apoptosis by releasing BIM from binding with prosurvival proteins. (A) The mRNA expression levels of BCL-2 family members in hypertrophic scar tissues and adjacent normal skin tissues, including prosurvival proteins (BCL-2, BCL-XL, and BCL-W), activators (BID and BIM), and effectors (BAX and BAK). n = 4. (B) The mRNA expression levels of BCL-2 family members in HFBs and HSFs, including BCL-2, BCL-XL, BCL-W, BID, BIM, BAX, and BAK. n = 3. (C) The protein expression levels of BCL-2, BCL-XL, and BIM in HFBs and HSFs were measured by western blotting. n = 3. (D) BIM is sequestered by BCL-XL and BCL-2 in HSFs and displaced by ABT-263. Anti-BIM immunoprecipitation of whole-cell lysates from HFBs and HSFs treated with or without ABT-263 for 8 h. BCL-2 and BCL-XL were immunoblotted. Data represented the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, and n.s., no significance.