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. 2021 Jan 14;11:615505. doi: 10.3389/fphar.2020.615505

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Copyright © 2021 Yang, Xiao, Zhong, Shu, Xiao, Zheng and Xia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ABT-263 reduced the expression of α -SMA, collagen I, and collagen III in HSFs. (A) The mRNA expression levels of α -SMA, collagen I, and collagen III in HSFs after treatment with different concentration of ABT-263 were determined by PCR. n = 3. (B) The protein expression levels of α-SMA, collagen I, and collagen III in ABT 263-treated HSFs were determined by western blot. n = 3. (C) The protein expression levels of collagen I and α-SMA in ABT-263 treated HSFs were further confirmed by immunofluorescence staining. Collagen I was stained with red, α-SMA with green, and nuclei with blue. Scale bars: 100 μm. Data represented the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, and n.s., no significance. vs. control.